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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Biblioteca (s) : |
INIA Las Brujas; INIA Tacuarembó. |
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Fecha : |
05/09/2014 |
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Actualizado : |
24/06/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
REYNO, R.; NARANCIO, R.; SPERANZA, P.; DO CANTO, J.; LOPEZ-CARRO, B.; HERNANDEZ, P.; BURGUEÑO, J.; REAL, D.; DALLA RIZZA, M. |
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Afiliación : |
RAFAEL ALEJANDRO REYNO PODESTA, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; RAFAEL NARANCIO FERES, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; JAVIER DO CANTO FAGUNDEZ, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; DANIEL REAL FERREIRO, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; MARCO DALLA RIZZA VILARO, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
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Título : |
Molecular and cytogenetic characterization of a collection of bahiagrass (Paspalum notatum Flügge) native to Uruguay. |
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Fecha de publicación : |
2012 |
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Fuente / Imprenta : |
Genetic Resources and Crop Evolution, 2012, v. 59, no.8, p. 1823-1832. |
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ISSN : |
0925-9864 |
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DOI : |
10.1007/s10722-012-9806-x |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 9 August 2011 / Accepted: 30 January 2012 / Published online: 22 February 2012. |
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Contenido : |
ABSTRACT.
Paspalum notatum is a subtropical grass present throughout America, and one of the main constituents of the natural grasslands in Uruguay. An
apomictic autotetraploid (2n = 4x = 40) is the most frequent cytotype. The occurrence of sexual diploids (2n = 2x = 20) has also been reported as well as the occasional presence of apomictic triploids and pentaploids in Argentina. In this study, ISSR (inter simple sequence repeats) molecular markers were used to analyze the genetic variability of 210 P. notatum individuals from a collection from Uruguay. Cytometric analyses and chromosome counts were used to assess the ploidy level of the individuals. All plants from Uruguay analyzed were tetraploid. Intra- and inter-population variability was found both in genomic DNA content and at the genotypic level. Several multilocus genotypes were shared among individuals within populations and among populations over moderate geographical ranges, at the same time, very dissimilar genotypes were found within the same population. Part of the genetic variance among populations can be explained by a broader scale geographic structure which is partly coincident with the traditionally recognized grassland management regions. In spite of the apparently high degree of genetic admixture within populations, groups of related genotypes seem to follow a broader geographical structure in the area under study. These results suggest that an efficient collection strategy for this apomictically reproducing species should include carefully planned intra- and inter-population sampling. A broader scale regional sampling strategy should also be considered although further studies will be required to define genetic structure at this level.
© 2012 Springer Science+Business Media B.V. MenosABSTRACT.
Paspalum notatum is a subtropical grass present throughout America, and one of the main constituents of the natural grasslands in Uruguay. An
apomictic autotetraploid (2n = 4x = 40) is the most frequent cytotype. The occurrence of sexual diploids (2n = 2x = 20) has also been reported as well as the occasional presence of apomictic triploids and pentaploids in Argentina. In this study, ISSR (inter simple sequence repeats) molecular markers were used to analyze the genetic variability of 210 P. notatum individuals from a collection from Uruguay. Cytometric analyses and chromosome counts were used to assess the ploidy level of the individuals. All plants from Uruguay analyzed were tetraploid. Intra- and inter-population variability was found both in genomic DNA content and at the genotypic level. Several multilocus genotypes were shared among individuals within populations and among populations over moderate geographical ranges, at the same time, very dissimilar genotypes were found within the same population. Part of the genetic variance among populations can be explained by a broader scale geographic structure which is partly coincident with the traditionally recognized grassland management regions. In spite of the apparently high degree of genetic admixture within populations, groups of related genotypes seem to follow a broader geographical structure in the area under study. These results suggest that an efficient collection strategy for this apomictically reprod... Presentar Todo |
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Thesagro : |
MARCADORES GENÉTICOS; PASPALUM NOTATUM; PLANTAS FORRAJERAS. |
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Asunto categoría : |
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F30 Genética vegetal y fitomejoramiento |
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Marc : |
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022 $a0925-9864
024 7 $a10.1007/s10722-012-9806-x$2DOI
100 1 $aREYNO, R.
245 $aMolecular and cytogenetic characterization of a collection of bahiagrass (Paspalum notatum Flügge) native to Uruguay.
260 $c2012
500 $aArticle history: Received: 9 August 2011 / Accepted: 30 January 2012 / Published online: 22 February 2012.
520 $aABSTRACT. Paspalum notatum is a subtropical grass present throughout America, and one of the main constituents of the natural grasslands in Uruguay. An apomictic autotetraploid (2n = 4x = 40) is the most frequent cytotype. The occurrence of sexual diploids (2n = 2x = 20) has also been reported as well as the occasional presence of apomictic triploids and pentaploids in Argentina. In this study, ISSR (inter simple sequence repeats) molecular markers were used to analyze the genetic variability of 210 P. notatum individuals from a collection from Uruguay. Cytometric analyses and chromosome counts were used to assess the ploidy level of the individuals. All plants from Uruguay analyzed were tetraploid. Intra- and inter-population variability was found both in genomic DNA content and at the genotypic level. Several multilocus genotypes were shared among individuals within populations and among populations over moderate geographical ranges, at the same time, very dissimilar genotypes were found within the same population. Part of the genetic variance among populations can be explained by a broader scale geographic structure which is partly coincident with the traditionally recognized grassland management regions. In spite of the apparently high degree of genetic admixture within populations, groups of related genotypes seem to follow a broader geographical structure in the area under study. These results suggest that an efficient collection strategy for this apomictically reproducing species should include carefully planned intra- and inter-population sampling. A broader scale regional sampling strategy should also be considered although further studies will be required to define genetic structure at this level. © 2012 Springer Science+Business Media B.V.
650 $aMARCADORES GENÉTICOS
650 $aPASPALUM NOTATUM
650 $aPLANTAS FORRAJERAS
700 1 $aNARANCIO, R.
700 1 $aSPERANZA, P.
700 1 $aDO CANTO, J.
700 1 $aLOPEZ-CARRO, B.
700 1 $aHERNANDEZ, P.
700 1 $aBURGUEÑO, J.
700 1 $aREAL, D.
700 1 $aDALLA RIZZA, M.
773 $tGenetic Resources and Crop Evolution, 2012$gv. 59, no.8, p. 1823-1832.
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Registro original : |
INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
22/01/2020 |
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Actualizado : |
22/01/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
SILVA, P.E.; RIET-CORREA, F.; COELHO, A.C.B.; ECHENIQUE, J.V.Z.; PEREIRA, C.M.; LIMA, M.; DIEL, D.G.; SCHILD, A.L. |
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Afiliación : |
P. ESTIMA-SILVA, Programa de pós-graduação, Faculdade de Veterinária, Universidade Federal de Pelotas - (UFPel), Pelotas, RS, Brazil; FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; A.C.B. COELHO, Programa de pós-graduação, Faculdade de Veterinária, Universidade Federal de Pelotas - (UFPel), Pelotas, RS, Brazil; J.V.Z. ECHENIQUE, Programa de pós-graduação, Faculdade de Veterinária, Universidade Federal de Pelotas - (UFPel), Pelotas, RS, Brazil; C. MARCOLONGO-PEREIRA, Faculdade de Veterinária, Centro Universitário do Espírito Santo (UNESC), Colatina, ES, Brazil; M. LIMA, Faculdade de Veterinária (FV), Universidade Federal de Pelotas (UFPel), Pelotas, RS, Brazil; D.G. DIEL, South Dakota State University, Brookings, Brazil; A.L. SCHILD, Faculdade de Veterinária (FV), Universidade Federal de Pelotas (UFPel), Pelotas, RS, Brazil. |
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Título : |
Identification of equine herpesvirus type 1 as cause of abortion in mares in Southern Brazil. (Communication). [Identificação do herpesvirus equino tipo 1 como causa de abortos em éguas no sul do Brasil]. (Comunicação) |
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Fecha de publicación : |
2019 |
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Fuente / Imprenta : |
Arquivo Brasileiro de Medicina Veterinaria e Zootecnia, 2019, Volume 71, Issue 4, Pages 1421-1424. OPEN ACCESS. Doi: 10.1590/1678-4162-10430 |
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ISSN : |
0102-0935 |
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DOI : |
10.1590/1678-4162-10430 |
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Idioma : |
Inglés |
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Notas : |
Article history: Recebido em 20 de dezembro de 2017 / Aceito em 6 de outubro de 2018. |
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Contenido : |
RESUMO.
Foi realizado um estudo retrospectivo dos casos suspeitos de aborto por herpesvírus equino observados na região sul do Rio Grande do Sul entre 1978 e 2016. Foram revisados os protocolos de necropsia do Laboratório Regional de Diagnóstico da Faculdade de Veterinária da Universidade Federal de Pelotas resgatando-se os dados epidemiológicos, lesões macroscópicas e histológicas de cada caso. Foram observados dois surtos da enfermidade com prevalência entre 5,7% e 50% nos diferentes
estabelecimentos, e dois casos individuais. Em todos os casos foram enviados fragmentos de órgãos fetais formolizados. Histologicamente, em todos os casos foram observados focos de necrose no fígado, pulmão e baço e presença de corpúsculos de inclusão acidofílico em hepatócitos, células epiteliais pulmonares e leucócitos. A imuno-histoquímica utilizando anticorpo policlonal comercial para herpesvirus equino-1 (EHV-1) revelou marcação positiva em todos os casos. Além disso, foi extraído
DNA dos tecidos emblocados em parafina dos casos e submetidos à técnica de nested-PCR seguida de sequenciamento genômico dos amplicons em duas amostras. Estes achados indicam, que EHV-1 deve ser considerado como diagnóstico diferencial em casos de aborto em equinos no sul do Rio Grande do Sul. |
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Palabras claves : |
ABORTION; EQUINE HERPESVIRUS-1; HERPESVIRUS EQUINO-1; IMMUNOHISTOCHEMISTRY; IMUNO-HISTOQUÍMICA; INFECTION; PLATAFORMA SALUD ANIMAL. |
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Thesagro : |
ABORTO; PCR. |
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Asunto categoría : |
L73 Enfermedades de los animales |
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URL : |
http://www.scielo.br/pdf/abmvz/v71n4/0102-0935-abmvz-71-04-1421.pdf
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Marc : |
LEADER 02577naa a2200349 a 4500
001 1060663
005 2020-01-22
008 2019 bl uuuu u00u1 u #d
022 $a0102-0935
024 7 $a10.1590/1678-4162-10430$2DOI
100 1 $aSILVA, P.E.
245 $aIdentification of equine herpesvirus type 1 as cause of abortion in mares in Southern Brazil. (Communication). [Identificação do herpesvirus equino tipo 1 como causa de abortos em éguas no sul do Brasil]. (Comunicação)$h[electronic resource]
260 $c2019
500 $aArticle history: Recebido em 20 de dezembro de 2017 / Aceito em 6 de outubro de 2018.
520 $aRESUMO. Foi realizado um estudo retrospectivo dos casos suspeitos de aborto por herpesvírus equino observados na região sul do Rio Grande do Sul entre 1978 e 2016. Foram revisados os protocolos de necropsia do Laboratório Regional de Diagnóstico da Faculdade de Veterinária da Universidade Federal de Pelotas resgatando-se os dados epidemiológicos, lesões macroscópicas e histológicas de cada caso. Foram observados dois surtos da enfermidade com prevalência entre 5,7% e 50% nos diferentes estabelecimentos, e dois casos individuais. Em todos os casos foram enviados fragmentos de órgãos fetais formolizados. Histologicamente, em todos os casos foram observados focos de necrose no fígado, pulmão e baço e presença de corpúsculos de inclusão acidofílico em hepatócitos, células epiteliais pulmonares e leucócitos. A imuno-histoquímica utilizando anticorpo policlonal comercial para herpesvirus equino-1 (EHV-1) revelou marcação positiva em todos os casos. Além disso, foi extraído DNA dos tecidos emblocados em parafina dos casos e submetidos à técnica de nested-PCR seguida de sequenciamento genômico dos amplicons em duas amostras. Estes achados indicam, que EHV-1 deve ser considerado como diagnóstico diferencial em casos de aborto em equinos no sul do Rio Grande do Sul.
650 $aABORTO
650 $aPCR
653 $aABORTION
653 $aEQUINE HERPESVIRUS-1
653 $aHERPESVIRUS EQUINO-1
653 $aIMMUNOHISTOCHEMISTRY
653 $aIMUNO-HISTOQUÍMICA
653 $aINFECTION
653 $aPLATAFORMA SALUD ANIMAL
700 1 $aRIET-CORREA, F.
700 1 $aCOELHO, A.C.B.
700 1 $aECHENIQUE, J.V.Z.
700 1 $aPEREIRA, C.M.
700 1 $aLIMA, M.
700 1 $aDIEL, D.G.
700 1 $aSCHILD, A.L.
773 $tArquivo Brasileiro de Medicina Veterinaria e Zootecnia, 2019, Volume 71, Issue 4, Pages 1421-1424. OPEN ACCESS. Doi: 10.1590/1678-4162-10430
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